Coding

Part:BBa_K1315007:Design

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-26)

The BCAM0228 Response Regulator from Burkholderia


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 370
    Illegal NgoMIV site found at 598
    Illegal NgoMIV site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.


Source

The source of this part is Burkholderia cenocepacia J2315 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.


References